How Flow Cytometry Machines Work & How Excedr Saves You Time & Money
Below are the advantages of flow cytometer leasing:
- We enable you to make payments over time and remove the burdensome upfront cost of instrument purchases
- Payments may be 100% tax deductible*, which makes leasing even more low-cost
- It costs less to lease than to purchase equipment outright
- Our worry-free repair coverage reduces equipment downtime
- Excedr takes care of the administrative work related to equipment procurement and upkeep
- Using the cash saved through our program, labs are better able to reinvest in their core business and operations (staffing, inventory, marketing/sales, etc.)
*Please consult your tax advisor to determine the full tax implications of leasing equipment.
Flow cytometry is a powerful technique that simultaneously measures and analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. Flow cytometry is widely used for analyzing expression of cell surface and intracellular molecules, characterizing and defining different cell types in heterogeneous cell populations, assessing the purity of isolated subpopulations, and analyzing cell size and volume.
Over the past 30 years, flow cytometry has become an indispensible tool for many scientific researchers and clinicians. The evolving technology ensures its application in a number of fields, including cell biology, drug discovery, cancer research, neuroscience, stem cell research, pathology, immunology, hematology, plant biology, food science and marine biology.
Components & Benefits
A flow system is made up of three main components. The fluidics system transports particles in a sample stream to the interrogation point and takes away the waste. The optics system consists of lasers to illuminate the particles in the sample stream and optical filters to direct the resulting light signals to the appropriate detectors. The electronics system converts the light signals from the detectors into electronic signals that can be processed by the computer. For some instruments equipped with a sorting feature, the electronics system is also capable of initiating sorting decisions to charge and deflect particles. This is often referred to as fluorescence-actived cell sorting (FACS).
The Fluidics System
The fluidics system within the flow cytometer transports particles in a fluid stream to the laser beam for interrogation. When a sample in solution is injected into the instrument, the particles are randomly distributed in a three-dimensional space. For accurate data collection, it is important that the particles or cells are passed through the laser beam one at a time. Most flow cytometry instruments accomplish this by injecting the sample stream into a stream of faster flowing sheath fluid within the flow chamber. The flow of the sheath fluid accelerates the particles and restricts them to the center of the sample core, so that a single stream of particles is created. This process is known as hydrodynamic focusing.
The Optics System
After the process of hydrodynamic focusing, each particle passes through one or more beams of light. Light scattering, through a forward scatter channel (FSC) or fluorescence emission (if the particle is labeled with a fluorochrome), provides information about the particle’s properties. Cell granularity or internal structural complexity is provided via a side scatter channel (SSC). The laser and the arc lamp are the most popular light sources in modern flow cytometry.
Fluorescent measurements taken at different wavelengths can provide quantitative and qualitative data about fluorochrome-labeled cell surface receptors or intracellular molecules, such as DNA and cytokines. The argon ion laser is most commonly used in flow cytometry. This is because the 488 nm light that it emits excites more than one fluorochrome.
Ultimately the Excedr lease program can accommodate your lab’s instrument needs and brand/model preferences. Get in touch with us today and see how leasing can save you time and money.
The Electronics System
Once a cell or particle passes through the laser light, emitted side scatter and fluorescence signals are diverted to the photomultiplier tubes (PMTs) and a silicon photodiode collects the forward scatter signals. All of the signals are routed to their photodetectors via a system of mirrors and optical filters. Optical filters control the specificity of detection by blocking certain wavelengths, and transmitting others.
Most flow systems can utilize sample volumes ranging from several microliters to hundreds of microliters. If you have a small volume of precious cells, you may wish to consider using a microfluidics-based flow cytometry chip. The development of microfluidic, lab-on-a-chip (LOC), technologies is one of the most innovative and cost-effective approaches toward the advancement of cytometry.
Automated introduction of samples to the flow cytometer allows for walk-away operation to be performed on large numbers of samples, using a variety of formats, from single test tubes to multi-well plates. Single tubes are often contained within rectangular grid arrangements or carousel formats to match laboratory equipment used for sample preparation.
We Lease a Variety of FACS Machine Brands & Models
This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional cash savings.
If you recently purchased equipment, Excedr can offer you cash for your instrument and convert your purchase into a long-term rental. This is called a sale leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up cash in your budget rather than tying it down to the instrument.
Flow Cytometer Manufacturers & Models on the Market
- EMD Millipore: Muse, Guava easyCyte, Amnis, Scepter
- Beckman Coulter: AQUIOS CL, CytoFLEX, Cytomics FC 500, Gallios, Navios, MoFlo Astrios EQ, MoFlo XDP
- Bio-Rad: ZE5, S3e
- BD Bioscience: Accuri C6 Plus, FACSCelesta, FACSVerse, LSRFortessa, LSRFortessa X-20, FACSCanto II, FACSAria Fusion, Influx, FACSMelody, FACSAria III, FACSJazz, FACSCalibur, FACSCount, FACSVia, FACSCanto, FACSLyric, FACS Lyse, FACS Sample Prep Assistant (SPA III)
- Miltenyi-Biotech: MACSQuant Analyzer 10, MACSQuant VYB, MACSQuant X, MACSQuant Tyto
- ACEA Biosciences: NovoCyte, xCELLigence
- Thermo Fisher: Attune Nxt
- Cytonome: Viva, Hydris, Gigasort
- Sysmex Partec: CyFlow Cube 6, Cube 6 Max, CyFlow Cube 8, Cube 8 Max, CyFlow Ploidy, CyFlow Space
- and more!
Regardless if you are interested in leasing a PCR system or a cell sorter leaseback, we can help. Call us at (510) 982-6552 or fill in the contact form below to specify the equipment you need.
Leasing does not hinder your future borrowing ability and allows you to keep your business credit line open for expansions, staffing, and other operational expenses. Additionally, it strengthens the cash flow of your business and keeps cash reserves free for business development opportunities.
Unlike traditional financing and leasing companies, the Excedr program can accommodate refurbished/reconditioned equipment in addition to demo units. If you are looking for additional cost-savings, we recommend considering this option.
Excedr’s lease program allows you to respond quickly as your need for equipment and technology arises. You can be approved with minimal documentation and have the equipment you need in operation and generating revenue for your business quickly.