How High-Throughput Sequencing Works & How Our Services Save Time & Money
Below are the benefits of Excedr’s NGS leasing program:
- We enable you to spread out payments over time and remove the large upfront cost of equipment purchases
- Payments may be 100% tax deductible*, which yields you additional cost-savings
- Instrument downtime is minimized with our repair coverage
- Excedr handles the admin work associated with equipment procurement and upkeep
- Using the capital saved through our program, companies are better able to reinvest in their core business and operations (staffing, inventory, marketing/sales, etc.)
*Please consult your tax advisor to determine the full tax implications of leasing equipment.
NGS technology is evolving at an unprecedented speed. Scientists can now routinely examine a single genome a large number of times, observe individual changes, study population variations, study the microbiome and metagenomics, differentiate cancer genomes from healthy genomes, study the epigenome, and investigate the possibility of personalized medicine, among other applications.
There are several main suppliers of next generation sequencing instruments and they all share the same fundamental sequencing process, but with varying technologies. Regardless of their method of arrival, next generation sequencers rely on the generation of representative, unbiased sources of nucleic acid templates from the complex genomes being interrogated. Clonally amplified DNA templates, or single DNA molecules, are sequenced in a massively parallel fashion in a flow cell. The sequencing is conducted in either a stepwise iterative process or in a continuous real-time manner. In this way, the instruments allow for the sequencing of up to billions of individual DNA templates in a single reaction.
Methods & Benefits of Next Generation Sequencers
Semiconductor technology directly translates chemically encoded information (A, C, G, T) into digital information (0, 1) on a semiconductor chip, similar to the one you might find in your digital camera. Some systems essentially act as the world’s smallest solid-state pH meter to determine DNA sequences. The DNA is fragmented, attached to beads and deposited in millions of wells across the surface of the chip. The wells are then sequentially flooded with one nucleotide after another. If a nucleotide is incorporated into the strand of bead-bound DNA, a hydrogen ion is given off, a chemical change is measured by an ion sensor beneath the well, and a base is called. This process takes place in millions of wells simultaneously, enabling sequencing in only a few hours.
DNA ligase is used to determine the underlying sequence of the target DNA molecule. A fluorescently labeled di-based probe hybridizes to its complementary sequence adjacent to the primed template. The dye-labeled probe is then joined to the primer following the addition of DNA ligase. Non-ligated probes are washed away, and the ligated probe is identified using fluorescent imaging. Each base is effectively probed by two independent ligation reactions using two different primers.
Single Molecule, Real-Time Technology
SMRT technology, in which DNA polymerase attaches itself to a strand of DNA to be replicated, examines the individual base at the point it is attached, and then determines which of four building blocks, or nucleotides, is required to replicate that individual base. After determining which nucleotide is required, the polymerase incorporates that nucleotide into the growing strand that is being produced. After incorporation, the enzyme advances to the next base to be replicated and the process is then repeated.
No matter what your equipment needs might be, the Excedr lease program can procure virtually all equipment types and can accommodate any brand preferences your end-user might have. Drop us a line today and see how leasing can save you time and money.
Sequencing by Synthesis Technology
Sequencing templates are immobilized on a proprietary flow cell surface that is designed to present the DNA in a manner that facilitates access to enzymes, while ensuring high stability of surface bound template and low non-specific binding of fluorescently labeled nucleotides. Solid-phase amplification creates up to 1,000 identical copies of each single template molecule in close proximity, generating a cluster, and because this process does not involve positioning of beads into wells or mechanical spotting, much higher densities are achieved. SBS technology uses four fluorescently-labeled nucleotides to sequence the tens of millions of clusters on the flow cell surface in parallel, using a proprietary reversible terminator-based method. This enables detection of single bases as they are incorporated into growing DNA strands. Since all four reversible terminator-bound dNTPs are present during each sequencing cycle, natural competition minimizes incorporation bias. The result is base-by-base sequencing that enables highly accurate data for a broad range of applications.
Nanopore technology is an exciting new method currently being developed. The technology records characteristic changes in electric current as nucleic acids pass through a synthetic or protein nanopore and will theoretically allow sequencing of a complete chromosome in one step, without the need to generate a new DNA strand.
Lease a Cutting-Edge NGS System for Your Lab
This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional cash savings.
If you recently purchased equipment, Excedr can offer you cash for your instrument and convert your purchase into a long-term rental. This is called a sale leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up cash in your budget rather than tying it down to the instrument.
Next Generation Sequencer Manufacturers & Models on the Market
- Illumina – MiniSeq, MiSeq, NextSeq, HiSeq, HiSeq X, NovaSeq
- ThermoFisher & Life Technologies – Ion Torrent, Ion PGM, Ion Proton, Ion S5, Ion S5 XL
- Oxford Nanopore Technologies – MinION, PromethION, GridION X5, SmidgION
- Pacific Biosciences – PacBio RS II, PacBio Sequel System
- Qiagen – GeneReader NGS System, GeneRead, QIAseq
- Fluidigm – Juno
Regardless if you are looking to lease a flow cytometer or leaseback a next generation sequencer, we can help. Call us at (510) 982-6552 or fill out our contact form on the right-hand side to discuss your lab equipment needs.
Leasing does not hinder your future borrowing ability and allows you to keep your business credit line open for expansions, staffing, and other operational expenses. Additionally, it strengthens the cash flow of your business and keeps cash reserves free for business development opportunities.
Unlike traditional financing and leasing companies, the Excedr program can accommodate refurbished/reconditioned equipment in addition to demo units. If you are looking for additional cost-savings, we recommend considering this option.
Excedr’s lease program allows you to respond quickly as your need for equipment and technology arises. You can be approved with minimal documentation and have the equipment you need in operation and generating revenue for your business quickly.