How Spectrophotometry Works & How Our Program Saves You Time & Money

Below are the advantages of spectrophotomer leasing:

  • We enable you to make payments over time, which significantly reduces the upfront cost of equipment acquisition
  • You earn additional cost-savings since lease payments may be 100% tax deductible*
  • Instrument downtime is minimized with our all-inclusive repair coverage
  • Excedr streamlines the admin work associated with equipment procurement and maintenance
  • With the cash saved through our services, labs are better able to reinvest in their core business and operations (staffing, inventory, marketing/sales, etc.)

*Please consult your tax advisor to determine the full tax implications of leasing equipment.

In chemistry, spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. It is more specific than the general term electromagnetic spectroscopy in that it deals with visible light, near-ultraviolet, and near-infrared, but does not cover time-resolved spectroscopic techniques.

Spectrophotometry uses photometers that can measure a light beam’s intensity as a function of its color (wavelength). Important features of these instruments are spectral bandwidth (the range of colors it can transmit through the test sample), the percentage of sample-transmission, the logarithmic range of sample-absorption, and sometimes a percentage of reflectance measurement.

A common spectrophotometer use is for the measurement of transmittance or reflectance of solutions, transparent or opaque solids, such as polished glass, or gases. However they can also be designed to measure the diffusivity on any of the listed light ranges that usually cover around 200 nm – 2500 nm using different controls and calibrations. Within these ranges of light, calibrations are needed on the machine using standards that vary in type depending on the wavelength of the photometric determination.

An example of an experiment in which spectrophotometry is used is the determination of the equilibrium constant of a solution. A certain chemical reaction within a solution may occur in a forward and reverse direction where reactants form products and products break down into reactants. At some point, this chemical reaction will reach a point of balance called an equilibrium point. In order to determine the respective concentrations of reactants and products at this point, the light transmittance of the solution can be tested using spectrophotometry. The amount of light that passes through the solution is indicative of the concentration of certain chemicals that do not allow light to pass through.

Application & Types

The use of spectrophotometers spans various scientific fields, such as physics, materials science, chemistry, biochemistry, and molecular biology. They are widely used in many industries including semiconductors, laser and optical manufacturing, printing and forensic examination, as well in laboratories for the study of chemical substances. Ultimately these tools are able to determine, depending on the control or calibration, what substances are present in a target and exactly how much through calculations of observed wavelengths.

More specifically, spectrophotometry is an important technique used in many biochemical experiments that involve DNA, RNA, and protein isolation, enzyme kinetics and biochemical analyses. A brief explanation of the procedure  includes comparing the absorbency of a blank sample that does not contain a colored compound to a sample that contains a colored compound. The spectrophotometer is used to measure colored compounds in the visible region of light (between 350 nm and 800 nm), thus it can be used to find more information about the substance being studied. In biochemical experiments, a chemical and/or physical property is chosen and the procedure that is used is specific to that property in order to derive more information about the sample, such as the quantity, purity, enzyme activity, etc. It is also a helpful procedure for protein purification and can also be used as a method to create optical assays of a compound. Because the reader measures the wavelength of a compound through its color, a dye binding substance can be added so that it can undergo a color change and be measured. These instruments have been developed and improved over decades and have been widely used among chemists. It is considered to be a highly accurate instrument that is also very sensitive and therefore extremely precise, especially in determining color change. This method is also convenient for use in laboratory experiments because it is an inexpensive and relatively simple process.

UV-Vis
The most common spectrophotometers are used in the ultraviolet and visible regions of the spectrum, and some of these instruments also operate into the near-infrared region as well.

Visible region 400–700 nm spectroscopy is used extensively in colorimetry science. It is a known fact that it operates best at the range of 0.2-0.8 O.D. Ink manufacturers, printing companies, textiles vendors, and many more, need the data provided through colorimetry. They take readings in the region of every 5–20 nanometers along the visible region, and produce a spectral reflectance curve or a data stream for alternative presentations. These curves can be used to test a new batch of colorant to check if it makes a match to specifications, e.g., ISO printing standards.

Traditional visible region spectrophotometers cannot detect if a colorant or the base material has fluorescence. This can make it difficult to manage color issues if for example one or more of the printing inks is fluorescent. Where a colorant contains fluorescence, a bi-spectral fluorescent system is used. There are two major setups for visual spectrum machines, d/8 (spherical) and 0/45. The names are due to the geometry of the light source, observer and interior of the measurement chamber. Scientists use this instrument to measure the amount of compounds in a sample. If the compound is more concentrated more light will be absorbed by the sample; within small ranges, the Beer-Lambert law holds and the absorbance between samples vary with concentration linearly. In the case of printing measurements two alternative settings are commonly used- without/with UV filter to control better the effect of UV brighteners within the paper stock.

Samples are usually prepared in cuvettes; depending on the region of interest, they may be constructed of glass, plastic (visible spectrum region of interest), or quartz (far UV spectrum region of interest).

Additional applications of ultraviolet-visible spectroscopy include: Estimating dissolved organic carbon concentration, measuring specific ultraviolet absorption for metric of aromaticity, and Bial’s Test for concentration of pentoses.

Excedr is able to source all instrument types and can accommodate any model preferences your end-user might have. Request an estimate today and see how a lease can reduce your spectrophotometer price.

IR
The spectrophotometers designed for the infrared region are quite different because of the technical requirements of measurement in that region. One major factor is the type of photosensors that are available for different spectral regions, but infrared measurement is also challenging because virtually everything emits IR light as thermal radiation, especially at wavelengths beyond about 5 μm.

Another complication is that quite a few materials such as glass and plastic absorb infrared light, making it incompatible as an optical medium. Ideal optical materials are salts, which do not absorb strongly. Samples prepped for IR may be smeared between two discs of potassium bromide or ground with potassium bromide and pressed into a pellet. Where aqueous solutions are to be measured, insoluble silver chloride is used to construct the cell.

Customized Spectrophotomer Leases to Meet Your Needs

Operating Lease
This off-balance sheet financing structure provides three options at the end of the term. The lessee has the option to return the equipment to the lessor, renew at a discounted rate, or purchase the instrument for the fair market value. Monthly payments are also 100% tax deductible which yields additional cash savings.

Sale Leaseback
If you recently purchased equipment, Excedr can offer you cash for your instrument and convert your purchase into a long-term rental. This is called a sale leaseback. If you’ve paid for equipment within the last ninety days, we can help you recoup your investment and allow you to make low monthly payments. This also frees up cash in your budget rather than tying it down to the instrument.

Spectrophotometer Manufacturers & Models on the Market

  • Cole-Parmer

UV/VisEW-83059-10, EW-83059-15

VisibleEW-83055-10, EW-83055-20, EW-83055-30, EW-83057-01, EW-83057-06

Scanning UV/Visible Spectrometers: EW-83057-30, EW-83057-35, EW-83057-80, EW-83057-85

Spectrophotometer Sipper SystemEW-83057-66, EW-83057-68, EW-83057-70, EW-83057-74

Jenway: 6320 Benchtop Visible, EW-83054-00, EW-83054-07, 73 Series Visible and UV/Visible, EW-83058-15, EW-83058-16, EW-83058-17, EW-83058-18, 72 Series Visible and UV/Visible Diode Array Scanning, EW-83056-01, EW-83056-02, EW-83056-04, 67 Series Graphical Visible and UV/Visible Scanning, EW-83056-65, EW-83056-70, 74 Series Visible and UV/Visible Scanning with CPLive Connectivity, 7410 Scanning Visible, EW-83056-21, 7415 Scanning UV/Visible, EW-83056-22, 7415 Scanning Nano Micro-Volume, EW-83056-23, EW-83056-31, 741001B, EW-83056-32, 741501B, EW-83056-33, 747501B, 737-501 Life Science Analyzer, EW-83070-02, 736-501 Life Science Analyzer, EW-83070-00, 6850 Double-Beam, EW-83070-04, EW-83070-06, EW-83070-08, 6300 (630 501) Benchtop Visible, EW-83054-04

  • Implen: NanoPhotometer, Cuvette, NP80, N60, N50, C40, DiluPhotometer, OD600
  • Thermo Fisher Scientific: SPECTRONIC 200, GENESYS 10S Vis, VISIONlite 5, GENESYS 40 UV-Vis, GENESYS 40 Vis, GENESYS 10S UV-Vis, GENESYS 20 Visible, GENESYS 180 UV-Vis, GENESYS 30 Visible, GENESYS 140 Vis, GENESYS 150 UV-Vis, Evolution 300 Security UV-Vis, Evolution 300 PC UV-Vis, Evolution 350, Evolution 350 UV-Vis, Evolution 60S UV-Vis, Evolution 201 UV-Vis, Evolution 220 UV-Vis, Evolution 300 Security UV-Vis, Evolution 300 PC UV-Vis, BioMate 160 UV-Vis, NanoDrop One, NanoDrop One Microvolume UV-Vis, NanoDrop OneC Microvolume UV-Vis, NanoDrop One Microvolume UV-Vis, NanoDrop 8000 Microvolume UV-Vis, NanoDrop Lite UV-Vis, NanoDrop 2000, NanoDrop 2000c, Multiskan Sky
  • Shimadzu: SolidSpec-3700, SolidSpec-3700DUV, UV-3600 Plus UV-Vis-NIR, UV-2600, UV-2700, UV-1900 UV-VIS, UV-1800, UV-1280, BioSpec-nano Micro-volume UV-Vis, IRAffinity-1S, IRTracter-100, IRSpirit
  • PerkinElmer: LAMBDA 150, LAMBDA 265, LAMBDA 365, LAMBDA 465, LAMBDA 650, LAMBDA 750, LAMBDA 850, LAMBDA 950, LAMBDA Bio, LAMBDA Bio+, LAMBDA XLS, LAMBDA XLS+, FL 6500 Flourescence,  FL 8500 Flourescence
  • Eppendorf: BioPhotometer D30, BioSpectrometer basic, BioSpectrometer kinetic, BioSpectrometer fluorescence, µCuvette G1.0, BioSpectrometer Reference Filter Sets, BioSpectrometer fluorescence Reference Filter Sets
  • Cecil Instruments: Aurius – CE 2011, CE 2021, CE 2021D, System 1, Super Aurius – CE 3021, CE 3021D, System 1
  • Biotek: Epoch and PowerWave HT
  • Hach: DR1900 Portable, DR3900 Laboratory, DR6000 Laboratory UV/VIS
  • Biochrom: Libra S80 UV/VIS, Libra 280 PC, Libra S70, Libra S70PC, Libra S60 UV/Vis, Libra S60PC, Libra S50 UV/Vis, Libra S50PC UV-Vis, Libra S22 UV-Vis, Libra S21 Visible, Libra S6+ Visible, Libra S4+ Visible, WPA Biowave DNA, WPA Lightwave II, WPA Lightwave II+, WPA Biowave II, WPA Biowave II+, WPA S1200+ Visible, WPA S800+ Visible, WPA CO8000 Cell Density Meter, WPA CO07500 Colorwave Colorimeter, WPA CO7000 Colorwave Medical Colorimeter, QuantiMate Micro-Volume Cuvette
  • Agilent: Cary 7000 Universal Measurement Spectrophotometer (UMS), Cary 3500 UV-Vis, Cary 8454 UV-Vis Diode Array System, Cary 60 UV-Vis, Cary 5000 UV-Vis-NIR, Cary 300 UV-Vis, Cary 6000i UV-Vis-NIR, Cary 100 UV-Vis, Cary 4000 UV-Vis
  • Buck Scientific: Atomic Absorption (AA), 205 AA, 210VGP AA, 211 Accusys AA, 235ATS AA, 230ATS AA, 225ATS AA, M530 Quick-Scan Infrared, M530 IR
  • Mettler Toledo: UV5, UV7, UV, UV5Bio, UV5Nano
  • and more!
All equipment brands/models are available
Contact Us Today

No matter if you’re looking to lease a cryostat or leaseback a spectrophotomer, we can be of assistance. We want to provide you with exactly what you’re looking for, so call us at (510) 982-6552 or fill out the contact form to find out how we can help.

Effect On Credit & Operating Capital

Leasing/renting does not hinder your future borrowing ability and allows you to keep your business credit line open for expansions, staffing, and other operational expenses. Additionally, it strengthens the cash flow of your business and keeps cash reserves free for business development opportunities.

Used Equipment

Unlike traditional financing and leasing companies, the Excedr program can accommodate refurbished/reconditioned equipment in addition to demo units. If you are looking for additional cost-savings, we recommend considering this option.

Speed Of Approval

Excedr’s program allows you to respond quickly as your need for equipment and technology arises. You can be approved with minimal documentation and have the equipment you need in operation and generating revenue for your business quickly.

Request Lease Estimate

Your Full Name

Your Email Address

Phone Number

Brand of Instrument