ELISA, or enzyme-linked immunosorbent assay, is a plate-based assay technique used in analytical biochemistry and other fields of study to detect and quantify soluble substances, such as proteins, antibodies, and peptides in complex mixtures.Originally described by Engvall and Perlmann in 1971, ELISA enables the analysis of protein and other samples, which have been immobilized in microplate wells using specific antibodies. Immunosorbent assays are typically performed in 96-well or 384-well polystyrene plates that passively bind antibodies and proteins, are simple to design, and easy to perform.
August 28, 2023
What makes sandwich ELISA unique? Dive into this technique that employs two antibodies to detect target antigens and its applications.
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July 18, 2023
What is ELISA diluent and how does it work? Explore its mechanisms, roles, and applications in this article.
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June 7, 2023
ELISA plates are essential for immunoassays. Discover their working mechanisms, formats, and applications in detail.
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June 7, 2023
Learn about immunoassays, a vital biochemical technique to detect and quantify biomolecules via antibody-antigen interaction.
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May 4, 2023
Learn about ELISA substrates in our blog post. Dive into the world of signal amplification and visualization with hydrogen peroxide, luminol, and pNPP substrates. Explore their functions and applications in medical and biotech labs.
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April 3, 2023
ELISA assay is essential in life sciences labs for molecule analysis. Explore the four types: Direct, Indirect, Sandwich, and Competitive ELISA. Learn the steps, reagents, and kits to simplify your lab work and achieve accurate results.
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