ELISA, or enzyme-linked immunosorbent assay, is a plate-based assay technique used in analytical biochemistry and other fields of study to detect and quantify soluble substances, such as proteins, antibodies, and peptides in complex mixtures.Originally described by Engvall and Perlmann in 1971, ELISA enables the analysis of protein and other samples, which have been immobilized in microplate wells using specific antibodies. Immunosorbent assays are typically performed in 96-well or 384-well polystyrene plates that passively bind antibodies and proteins, are simple to design, and easy to perform.
June 30, 2022
Definition: Wash Buffer Lab experiments need to be carefully performed in order to achieve reliable and accurate results. Especially when it comes to high-throughput assays that require quality and ex...
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June 28, 2022
Definition: His-Tag Affinity tags are powerful tools used in life sciences labs for basic biological studies as well as structural and functional proteomics. The tags are structurally a short se...
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