PCR, qPCR, & DNA Amplification

A game that looks similar to Tetris. It represents dNTP, a biomolecule used to build nucleic acid.

Deoxynucleotide Triphosphates (dNTP): Definition & Overview

April 20, 2022

Deoxynucleotide Triphosphates (dNTP): Definition & Overview

What Is dNTP? Nucleoside triphosphate is a biomolecule involved in building nucleic acids (DNA and RNA). Structurally, they contain nitrogenous bases bound to a ribose sugar and three phosphates. And,...

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What Is Deoxyguanosine Triphosphate (dGTP)?

February 15, 2022

PCR, qPCR, & DNA Amplification

What Is Deoxyguanosine Triphosphate (dGTP)?

What is dGTP? Deoxyguanosine triphosphate (dGTP) is a nucleotide with a purine base (guanine), deoxyribose sugar, and three phosphate groups. It’s one of the four dNTPs (deoxynucleoside triphosphate) ...

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PCR Master Mix: Definition, Types, & More

February 15, 2022

PCR, qPCR, & DNA Amplification

PCR Master Mix: Definition, Types, & More

What is a Real-time PCR Master Mix? PCR master mix, as the name suggests, is a premixed solution of all reagents and essential components required to run a PCR assay. It contains dNTPs (dATP, dGTP, dT...

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What Is the Role of MgCl2 in PCR Amplification Reactions?

February 15, 2022

PCR, qPCR, & DNA Amplification

What Is the Role of MgCl2 in PCR Amplification Reactions?

Polymerase Chain Reaction (PCR) Basics Did you know Mullis and Michael Smith won the Nobel Prize for discovering the Polymerase Chain Reaction (PCR) in 1983? This groundbreaking technology-enabled sci...

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What Is dCTP? Definition & Use Cases

February 15, 2022

PCR, qPCR, & DNA Amplification

What Is dCTP? Definition & Use Cases

What is Deoxycytidine Triphosphate (dCTP)? 2′-deoxycytidine 5′-triphosphate (or dCTP) is a nucleoside triphosphate (a nucleotide), containing cytosine as its pyrimidine base. It also...

A puzzle that forms the shape of a DNA strand. Two pieces of the puzzle have been pulled out.

What Is Deoxythymidine Triphosphate (dTTP)?

February 15, 2022

PCR, qPCR, & DNA Amplification

What Is Deoxythymidine Triphosphate (dTTP)?

What is dTTP? 2′-deoxythymidine 5′-triphosphate (or dTTP) is one of the nucleotide bases among three other dNTPs (deoxynucleoside triphosphate)—dATP, dGTP, and dCTP—that are involved in th...