PCR, qPCR, & DNA Amplification

Polymerase chain reaction (PCR) is a rapid and inexpensive in vitro technique used to amplify copies of small segments of DNA, producing billions of DNA copies quickly and efficiently. Since its introduction in the 1980’s by Kary Mullis, it has significantly contributed to various innovations in molecular biology, biotechnology, and biopharmaceuticals, resulting in the development of numerous technologies.

PCR equipment and various symbols next to it
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The Basics of Reverse Transcription PCR (RT-PCR)

What is Reverse Transcription Polymerase Chain Reaction (RT-PCR)? Reverse transcription-polymerase chain reaction is a molecular technique commonly called RT-PCR. PCR stands for polymerase chain react...

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dATP: What Is Deoxyadenosine Triphosphate?

What is dATP? Deoxyadenosine triphosphate (dATP) is a nucleotide that is used as a building block in DNA synthesis, during both in vivo replication and transcription and in vitro replication technique...

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TAQ Polymerase: What Is It & What Does It Do?

Taq Polymerase Basics What is Taq Polymerase? Taq DNA Polymerase, or Taq polymerase, is a biological catalyst involved in the attachment of nucleotides to synthesize DNA––like any other polymerase. A ...

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DNA Polymerase: What Is It & What Does It Do?

What is DNA Polymerase? A number of molecular biologists originally believed that the first DNA molecule is the reason for life on earth. That sentiment changed after many years of research and experi...

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What Is Polymerase Chain Reaction (PCR)?

Effective research and advancements in life science would not be possible without the discovery of PCR. PCR has contributed greatly to innovations in biopharmaceuticals, biotech crops, and industrial ...