Western Blot

Western blot, or western blotting, is an analytical technique used to perform protein analysis. Specifically, western blotting, also referred to as immunoblotting, allows for the visualization and study of target proteins within a mixture of proteins.The technique involves using gel electrophoresis to separate the sample’s proteins, which are transferred out of the gel to the surface of a membrane. After, the membrane is exposed to an antibody that is specific to the target protein. The antibody binds to the target protein, and the binding is detected using labeled antibodies, or conjugated molecules.

4 Western Blotting Techniques for Accurate Results

September 29, 2023

Western Blot

4 Western Blotting Techniques for Accurate Results

Curious about the five types of western blotting methods? Explore this key immunoassay used in life sciences labs.

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Western blot vs. Souther blot vs. Northern blot

Western vs. Southern vs. Northern Blots: Key Differences

August 28, 2023

Western Blot

Western vs. Southern vs. Northern Blots: Key Differences

Curious about western, northern, and southern blotting? Discover their step-by-step procedures and applications in this article.

Protein Standards: Overview, Applications, & Industry Uses

July 18, 2023

Western Blot

Protein Standards: Overview, Applications, & Industry Uses

What role do protein standards play in research? Learn how they help determine the size and concentration of proteins in this article.

A results page displaying different types of Western Blot kits and buffers.

Western Blot Kits & Buffers: What’s Included & Common Uses

May 4, 2023

Western Blot

Western Blot Kits & Buffers: What’s Included & Common Uses

What are Western blot kits and how can they enhance your research? Explore their content, applications, and key considerations for selection.

A row of crosshatched squares ascending in order of size. Represents different types of Western Blot membranes.

Western Blot Membranes: Overview & Application

April 3, 2023

Western Blot

Western Blot Membranes: Overview & Application

Discover the right Western blot membrane for your research. Nitrocellulose and PVDF membranes compared. Learn about their properties and choose the ideal membrane for accurate protein detection and visualization in your experiments.

5 different symbols representing 5 types of Western Blot equipment.

5 Types of Western Blot Equipment: A Guide for Researchers

March 8, 2023

Western Blot

5 Types of Western Blot Equipment: A Guide for Researchers

Learn about Western blotting: a protein detection technique used in research labs. Explore the process, equipment needs, and applications. Leverage Excedr's leasing solution for high-quality equipment, saving your lab time and money.