ELISA, or enzyme-linked immunosorbent assay, is a plate-based assay technique used in analytical biochemistry and other fields of study to detect and quantify soluble substances, such as proteins, antibodies, and peptides in complex mixtures.Originally described by Engvall and Perlmann in 1971, ELISA enables the analysis of protein and other samples, which have been immobilized in microplate wells using specific antibodies. Immunosorbent assays are typically performed in 96-well or 384-well polystyrene plates that passively bind antibodies and proteins, are simple to design, and easy to perform.
August 28, 2023
Sandwich ELISA is a type of ELISA technique that uses two antibodies to analyze target antigens. Learn more about the technique and its applications here.
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July 18, 2023
ELISA diluent is a buffer solution used in ELISA assay to dilute samples. Explore more about its work mechanisms, roles, and applications in this article!
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June 7, 2023
Learn about the various types of immunoassays and their importance in the life sciences. Discover their working mechanisms and their role in clinical and diagnostic applications.
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June 7, 2023
ELISA plates play a crucial role in the success of enzyme-linked immunosorbent assays. In this comprehensive blog post, we delve into the features and formats of these microplates, exploring their applications across various industries.
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May 4, 2023
Learn about ELISA substrates in our blog post. Dive into the world of signal amplification and visualization with hydrogen peroxide, luminol, and pNPP substrates. Explore their functions and applications in medical and biotech labs.
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April 3, 2023
ELISA assay is essential in life sciences labs for molecule analysis. Explore the four types: Direct, Indirect, Sandwich, and Competitive ELISA. Learn the steps, reagents, and kits to simplify your lab work and achieve accurate results.
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