Enzyme Linked-Immunosorbent Assay (ELISA)

ELISA, or enzyme-linked immunosorbent assay, is a plate-based assay technique used in analytical biochemistry and other fields of study to detect and quantify soluble substances, such as proteins, antibodies, and peptides in complex mixtures.Originally described by Engvall and Perlmann in 1971, ELISA enables the analysis of protein and other samples, which have been immobilized in microplate wells using specific antibodies. Immunosorbent assays are typically performed in 96-well or 384-well polystyrene plates that passively bind antibodies and proteins, are simple to design, and easy to perform.

A field of orange flowers. There is one flower that is yellow. It represents how ELISA detection works.

ELISA Detection: Types, Methods, & More

March 8, 2023

ELISA Detection: Types, Methods, & More

Explore the ins and outs of ELISA detection. In this blog post, we review what is ELISA, ELISA detection, the types of ELISA, and methods of detecting the presence of an antigen or antibody. Read on to learn more.

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ELISA Blocking Buffers: Overview & Application

March 8, 2023

Enzyme Linked-Immunosorbent Assay (ELISA)

ELISA Blocking Buffers: Overview & Application

ELISA blocking buffers prevent non-specific binding, ensuring accurate results. They improve signal-to-noise ratio and are crucial for ELISA assays. Learn how to choose the right blocking buffer to optimize your immunoassays.

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ELISA Antibodies: Overview & Applications

February 7, 2023

Enzyme Linked-Immunosorbent Assay (ELISA)

ELISA Antibodies: Overview & Applications

ELISA: Overview Enzyme-linked immunosorbent assay (ELISA) antibodies are crucial elements of the ELISA assay that are used to study proteins. Before proceeding to understand the antibody roles, it’s e…

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Enzyme-Linked Immunosorbent Assay: Overview & Application

January 18, 2023

Enzyme Linked-Immunosorbent Assay (ELISA)

Enzyme-Linked Immunosorbent Assay: Overview & Application

Enzyme-Linked Immunosorbent Assay: Overview Enzyme-linked immunosorbent assay, or ELISA, is a plate-based biochemical assay used in labs to detect and quantify substances in a complex mixture, such as...

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Cell Lysate: Definition, Applications, & Industry Uses

July 12, 2022

Enzyme Linked-Immunosorbent Assay (ELISA)

Cell Lysate: Definition, Applications, & Industry Uses

Cell lysate is the preparation obtained after lysing a cell population in labs using certain chemical reagents and enzymes, or by osmotic and mechanical disruption. They ca...

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GST Tag: Definition, Applications, & Industry Uses

July 1, 2022

Enzyme Linked-Immunosorbent Assay (ELISA)

GST Tag: Definition, Applications, & Industry Uses

Definition: GST-tagged Proteins Protein affinity tags are special proteins or peptides added to the C- or N-terminus of recombinant proteins for their easy and targeted purification. Many protein affi...