Western Blot

Western blot, or western blotting, is an analytical technique used to perform protein analysis. Specifically, western blotting, also referred to as immunoblotting, allows for the visualization and study of target proteins within a mixture of proteins.The technique involves using gel electrophoresis to separate the sample’s proteins, which are transferred out of the gel to the surface of a membrane. After, the membrane is exposed to an antibody that is specific to the target protein. The antibody binds to the target protein, and the binding is detected using labeled antibodies, or conjugated molecules.

4 Western Blotting Techniques for Accurate Results

September 29, 2023

Western Blot

4 Western Blotting Techniques for Accurate Results

Interested in learning the five types of western blotting methods? Read more about this immunoassay used in life sciences labs today.

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Western blot vs. Souther blot vs. Northern blot

Western vs. Southern vs. Northern Blots: Key Differences

August 28, 2023

Western Blot

Western vs. Southern vs. Northern Blots: Key Differences

Interested in learning about the western, northern, and southern blotting methods? This article presents an overview of their step-wise procedure and uses

Protein Standards: Overview, Applications, & Industry Uses

July 18, 2023

Western Blot

Protein Standards: Overview, Applications, & Industry Uses

Discover the role of protein standards in research and analysis and learn how they aid in determining the size and concentration of proteins in this blog.

A results page displaying different types of Western Blot kits and buffers.

Western Blot Kits & Buffers: What’s Included & Common Uses

May 4, 2023

Western Blot

Western Blot Kits & Buffers: What’s Included & Common Uses

Unlock the power of Western blotting with the right kits and buffers. Explore the importance of optimized buffers and choose from a wide range of Western blot kits to enhance protein detection, visualization, and quantification in your research.

A row of crosshatched squares ascending in order of size. Represents different types of Western Blot membranes.

Western Blot Membranes: Overview & Application

April 3, 2023

Western Blot

Western Blot Membranes: Overview & Application

Discover the right Western blot membrane for your research. Nitrocellulose and PVDF membranes compared. Learn about their properties and choose the ideal membrane for accurate protein detection and visualization in your experiments.

5 different symbols representing 5 types of Western Blot equipment.

5 Types of Western Blot Equipment: A Guide for Researchers

March 8, 2023

Western Blot

5 Types of Western Blot Equipment: A Guide for Researchers

Learn about Western blotting: a protein detection technique used in research labs. Explore the process, equipment needs, and applications. Leverage Excedr's leasing solution for high-quality equipment, saving your lab time and money.