Western Blot

Western blot, or western blotting, is an analytical technique used to perform protein analysis. Specifically, western blotting, also referred to as immunoblotting, allows for the visualization and study of target proteins within a mixture of proteins.The technique involves using gel electrophoresis to separate the sample’s proteins, which are transferred out of the gel to the surface of a membrane. After, the membrane is exposed to an antibody that is specific to the target protein. The antibody binds to the target protein, and the binding is detected using labeled antibodies, or conjugated molecules.

The words "Western Blot" written out on a spreadsheet by coloring different cells blue.

How To Read a Western Blot: A Guide to Understanding Results

February 7, 2023

Western Blot

How To Read a Western Blot: A Guide to Understanding Results

Introduction Western blotting is a powerful technique used in molecular biology and proteomics labs to study proteins obtained from a cell lysate or other protein samples. It’s a powerful procedure to…

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An illustration of the results of a Western Blot.

Western Blot: Overview & Applications

January 18, 2023

Western Blot

Western Blot: Overview & Applications

Western Blot: Overview Western blotting is a technique used in labs to separate and identify specific proteins in tissue, blood samples, or other cell lysates. It’s also known as immunoblotting. It in...

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Secondary Antibody: Overview & Definition

July 12, 2022

Western Blot

Secondary Antibody: Overview & Definition

Overview of Secondary Antibodies Antibodies (also known as immunoglobulin) play a crucial role in life science, research, and diagnostic centers to diagnose diseases and develop targeted drugs & t...

An abstract drawing of shapes. Represents primary antibodies.

Primary Antibody: Definition & Applications

July 12, 2022

Western Blot

Primary Antibody: Definition & Applications

Definition: Primary Antibody Antibodies are small protective proteins produced by our immune system in response to disease or the presence of an antigen. It is also known as immunoglobulin. In m...

Differently colored shapes. It represents the different types of cell membranes.

Membrane: Definition, Applications, & Industry Uses

June 29, 2022

Western Blot

Membrane: Definition, Applications, & Industry Uses

Definition: Membrane A cell membrane is a thin layer present around living cells (also known as the biological membrane or plasma membrane) and some organelles such as mitochondria, nucleus, and chlor...